Katie Fisher stands in front of the HI-SEAS habitat in an EVA suit with Mauna Kea in the background. BioNutrients-3 Kefir Growth Experiment Completed During Analog Astronaut Mission From March 4 to 9 at the Hawaiian Space Exploration Analog and Simulation (HI-SEAS) located on Mauna Loa volcano on the Big Island, NASA Ames Scientist Katie Fisher participated as Mission Commander for the 6-day lunar analog. During the mission, she collaborated with the Synthetic Biology BioNutrients team to test continuous passaging and growth methods of BioNutrients-3 kefir cultures.
The mission was a great learning experience for the team of five international analog astronauts. They worked together to overcome connectivity issues and a power outage while still completing experiments, reports, and medical evaluations.
By successfully accomplishing the kefir passaging experiment the team has demonstrated the ability to produce daily fresh cultures of kefir that will provide future astronauts valuable probiotic cultures and nutrients. Overall, the experiment was simple to execute with minimal resources and time. The pH indicator and color board allowed the crew to easily determine when the culture had reached the optimal pH. All 15 experimental bags were shipped back to Ames and are pending analysis of pH, viability, and contamination checks.
Analog Astronauts Katie Fisher and Tuğcağ Dumlupinar of Turkey perform bag hydration and passaging step of kefir cultures. Top right: Pre-incubation. Bottom right: 24 h post-incubation. Pictures courtesy of Katie Fisher.
